Composite

Part:BBa_K2057009:Design

Designed by: Jos� Eleazar Barboza Corona   Group: iGEM16_Guanajuato_Mx   (2016-10-17)


Alginate lyase controlled by lasI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No comments


Source

Alginate lyase come from the chromosome of Bacillus thuringiensis 4Q7, and lasI promoter from BBa_K649000

References

(1) Farrel EK, Tipton PA (2012) Functional characterization of AlgL, an alginate lyase from Pseudomona aeruginosa. Biochem. 51:10259-10266.

(2) Saeidi N, Wong CK, Lo TM, Nguyen HX, Ling H, Leong SS, Poh CL, Chang MW (2011) Engineering microbes to sense and eradicate Pseudomonas aeruginosa, a human pathogen. Mol Syst Biol. 7:521

(3) Barboza Corona JE, Nieto-Mazzocco E, Velázquez-Robledo R, Salcedo-Hernandez R, Bautista M, Jiménez B, Ibarra JE (2003) Cloning, sequencing and expression of the chitinase chiA74 from Bacillus thuringiensis. Appl Environ Microbiol. 2003 Feb;69(2):1023-1029.